Nerve Growth Factor Kit ELISA

N° du produit ABIN2044061

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Singe Nerve Growth Factor.

Aperçu rapide pour Nerve Growth Factor Kit ELISA (ABIN2044061)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Singe

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Détail du produit Nerve Growth Factor Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Marque: BioAssay™

Sensibilité: < 6.2 pg/mL

Ingrédients: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Information d'application

Plaque: Pre-coated

Protocole: The Rhesus Monkey Nerve Growth Factor (NGF) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NGF in simian serum, plasma, tissue homogenates, cell lysates and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NGF in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Nerve Growth Factor (NGF)

Pathways: Regulation of Cell Size